Journal: The Journal of Neuroscience

Article Title: Glutamate Transporter GLAST Is Expressed in the Radial Glia–Astrocyte Lineage of Developing Mouse Spinal Cord

doi: 10.1523/JNEUROSCI.17-23-09212.1997

Figure Lengend Snippet: Double-labeling showing morphological and immunochemical properties of GLAST-expressing cells at E13 (A, B), E15 (C, D), and P7 (E–G). A, GLAST immunoreactivity (green) and nuclear staining with propidium iodide (red). B–D, GLAST immunoreactivity (green) and nestin immunoreactivity (red) in the marginal zone (B, D) and mantle zone (C).Arrowheads indicate GLAST-immunopositive punctate or irregular protrusions from radial processes. Arrowsindicate conical endfeet of radial processes. E, GLAST mRNA (purple) and GFAP immunoreactivity (brown). Arrows indicate cell bodies labeled for the GLAST mRNA. F, GLAST immunoreactivity (green) and GFAP immunoreactivity (red). G, GLAST immunoreactivity (green) and synaptophysin immunoreactivity (red). Mg, Marginal zone;Mn, mantle zone; N, neuronal cell body;P, pial surface; Vn, ventricular zone; *, central canal. Scale bars, A–D, F, 10 μm; E, 50 μm; G, 5 μm.

Article Snippet: For double-labeling immunofluorescence, paraffin sections were incubated overnight at room temperature with guinea pig anti-GLAST antibody (2.5 μg/ml) and with rabbit anti-nestin antibody (1:500) ( Tomooka et al., 1993 ), rabbit anti-GFAP antibody (1:1), or rabbit anti-synaptophysin antibody (1:200) (Biotechnik, Heidelberg, Germany), respectively.

Techniques: Labeling, Expressing, Staining